L-aspartate + ATP → L-aspartyl-4-phosphate + ADP (R295)
- Feedback insensitive
  - Genome
  - Plasmid
- Overexpression
  - Genome
L-homoserine + NAD(P)+ ← L-aspartate 4-semialdehyde + NAD(P)H + H⁺ (R234)
- Feedback insensitive
  - Genome
  - Plasmid
- Overexpression
  - Genome
L-homoserine + ATP → O-phospho-L-homoserine + H⁺ + ADP (R153)
- Overexpression
  - Genome
  - Plasmid
H₂O + O-phospho-L-homoserine → L-threonine + phosphate (R646)
- Overexpression
  - Genome
  - Plasmid
H⁺ + meso-diaminopimelate → L-lysine + CO₂ (R367)
- Silencing
  - Genome
L-homoserine + succinyl-CoA → coenzyme A + O-succinyl-L-homoserine (R369)
- Silencing
  - Genome
L-threonine → 2-oxobutanoate + ammonium (R1249)
NAD⁺ + L-threonine → H⁺ + L-2-amino-3-oxobutanoate + NADH (R915)
- Silencing
  - Genome
phosphate + oxaloacetate ↔ phosphoenolpyruvate + hydrogencarbonate (R390)
- Overexpression
  - Genome
2-oxobutanoate + pyruvate + H⁺ → (S)-2-aceto-2-hydroxybutanoate + CO₂ (R277)
- Feedback insensitive
  - Plasmid
L-isoleucine → L-isoleucine (R00850)
- Overexpression
  - Plasmid
2-oxoglutarate + L-isoleucine ↔ (S)-3-methyl-2-oxopentanoate + L-glutamate (R758)
- Overexpression
  - Genome
    - Escherichia coli ILE02 (pTacilvAIH, pBRthrABCygaZH)
    - Escherichia coli ILE03 (pTacilvAIH, pBRthrABCygaZH)
(2R)-2,3-dihydroxy-3-methylbutanoate → H₂O + 3-methyl-2-oxobutanoate (R1255)
- Overexpression
  - Genome
    - Escherichia coli ILE02 (pTacilvAIH, pBRthrABCygaZH)
    - Escherichia coli ILE03 (pTacilvAIH, pBRthrABCygaZH)
(R)-2,3-dihydroxy-3-methylpentanoate + NADP⁺ ↔ NADPH + H⁺ + (S)-2-aceto-2-hydroxybutanoate (R93)
- Overexpression
  - Genome
    - Escherichia coli ILE02 (pTacilvAIH, pBRthrABCygaZH)
    - Escherichia coli ILE03 (pTacilvAIH, pBRthrABCygaZH)