pA-aroG^fbr-SeTAL-tyrA^fbr

Ancestors

All plasmids

pACYC184

pACYCDuet-1

pA-aroG^fbr-SeTAL-tyrA^fbr

Main features

TAL_Se, aroG^fbr_Ec, tyrA^fbr_Ec

Selection marker:

Chloramphenicol

Modifications

Gene	Organism	Modification
*sam8*	Saccharothrix espanaensis	Overexpression
*aroG*	Escherichia coli	Feedback insensitive
*tyrA*	Escherichia coli	Feedback insensitive

Strains harboring plasmid pA-aroG^fbr-SeTAL-tyrA^fbr

References

Lee, Hyejin; Kim, Bong Gyu; Kim, Mihyang & Ahn, Joong-Hoon (2015). Biosynthesis of Two Flavones, Apigenin and Genkwanin, in Escherichia coli. Journal of Microbiology and Biotechnology, 25(9), 1442-1448.
Lee, Su Jin; Sim, Geun Young; Kang, Hyunook; Yeo, Won Seok; Kim, Bong-Gyu & Ahn, Joong-Hoon (2018). Synthesis of avenanthramides using engineered Escherichia coli. Microbial Cell Factories, 17(1).