pA-aroG^fbr-ppsA-tktA-SeTAL-tyrA^fbr

Ancestors

All plasmids

pACYC184

pACYCDuet-1

pA-aroG^fbr-ppsA-tktA-SeTAL-tyrA^fbr

Main features

TAL_Se, aroG^fbr, ppsA, tktA, tyrA^fbr

Selection marker:

Chloramphenicol

Modifications

Gene	Organism	Modification
*sam8*	Saccharothrix espanaensis	Overexpression
*aroG*	Escherichia coli	Feedback insensitive
*ppsA*	Escherichia coli	Overexpression
*tktA*	Escherichia coli	Overexpression
*tyrA*	Escherichia coli	Feedback insensitive

Strains harboring plasmid pA-aroG^fbr-ppsA-tktA-SeTAL-tyrA^fbr

Escherichia coli HA-5

References

Lee, Su Jin; Sim, Geun Young; Kang, Hyunook; Yeo, Won Seok; Kim, Bong-Gyu & Ahn, Joong-Hoon (2018). Synthesis of avenanthramides using engineered Escherichia coli. Microbial Cell Factories, 17(1).