Ancestors

Derived strains

• Escherichia coli ΔcysK/pDES/pCA24N
• Escherichia coli ΔcysK/pDES/pGrx1
• Escherichia coli ΔcysK/pDES/pNrdH

Genotype with respect to parental

cysK::Km^R

Genotype with respect to wild type

F⁺ (λ) | IacI^q rrnB_T14 ∆lacZ_WJ16 hsdR514 ∆araBAD_AH33 ∆rhaBAD_LD78 | cysK::Km^R

Bars (|) indicate differences between strains.

References

• Tomoya Baba, Takeshi Ara, Miki Hasegawa, Yuki Takai, Yoshiko Okumura, Miki Baba, Kirill A Datsenko, Masaru Tomita, Barry L Wanner & Hirotada Mori (2006). Construction of Escherichia coli K‐12 in‐frame, single‐gene knockout mutants: the Keio collection. Molecular Systems Biology.

• Takeshi Nakatani, Iwao Ohtsu, Gen Nonaka, Natthawut Wiriyathanawudhiwong, Susumu Morigasaki & Hiroshi Takagi (2012). Enhancement of thioredoxin/glutaredoxin-mediated L-cysteine synthesis from S-sulfocysteine increases L-cysteine production in Escherichia coli. Microbial Cell Factories.

• Kawano, Yusuke; Onishi, Fumito; Shiroyama, Maeka; Miura, Masashi; Tanaka, Naoyuki; Oshiro, Satoshi; Nonaka, Gen; Nakanishi, Tsuyoshi & Ohtsu, Iwao. Improved fermentative l-cysteine overproduction by enhancing a newly identified thiosulfate assimilation pathway in Escherichia coli. Applied Microbiology and Biotechnology. 2017, 101(18), 6879-6889.