Escherichia coli TRP4

Ancestors

Genotype with respect to parental

ΔldhA ΔpoxB ΔpflB Δpta-ackA ΔadhE

Genotype with respect to wild type

F⁺ (λ) | λ- | inv(rrnD-rrnE) | F-, λ-, rph-1, IN (rrnD, rrnE) | ΔtrpR ΔtnaA ΔpheA ΔtyrA | Plasmid ColE-trpE*DBCA added | ΔldhA ΔpoxB ΔpflB Δpta-ackA ΔadhE

Bars (|) indicate differences between strains.

Plasmids

• ColE-trpE*DBCA (ColE1 Ori, P_tac-trpE^fbrDCBA)

References

• Guo, L., Ding, S., Liu, Y., Gao, C., Hu, G., Song, W., Liu, J., Chen, X., & Liu, L. (2022). Enhancing tryptophan production by balancing precursors in Escherichia coli. Biotechnology and Bioengineering, 119, 983–993.