Escherichia coli ΔcysM/pDES/pGlpE
Ancestors
Genotype with respect to wild type
F+ (λ) | IacIq rrnBT14 ∆lacZWJ16 hsdR514 ∆araBADAH33 ∆rhaBADLD78 | cysM::KmR | Plasmid pDES added | Plasmid pGlpE addedBars (|) indicate differences between strains.
Plasmids
Modifications
| Origin | Gene | Organism | Modification |
|---|---|---|---|
|
Silencing
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Feedback insensitive
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Overexpression
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Feedback insensitive
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Overexpression
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Production
| Metabolites | Production type | Production | Biomass | Carbon source | Time | Scale | Ref. |
|---|---|---|---|---|---|---|---|
| L-cysteine | Titer | 135.68 mg/L * | 72 h * | Flask | [319] | ||
| L-cysteine | Titer | 111.83 mg/L * | 72 h * | Flask | [319] |
* Inferred from plots using RetroPlot.
Heterologous.
Target metabolites are shown in bold, while non-bold metabolites represent intermediates or potential byproducts.
References
- Kawano, Yusuke; Onishi, Fumito; Shiroyama, Maeka; Miura, Masashi; Tanaka, Naoyuki; Oshiro, Satoshi; Nonaka, Gen; Nakanishi, Tsuyoshi & Ohtsu, Iwao. Improved fermentative l-cysteine overproduction by enhancing a newly identified thiosulfate assimilation pathway in Escherichia coli. Applied Microbiology and Biotechnology. 2017, 101(18), 6879-6889.