Escherichia coli PHE01

Strain derived from a randomly mutated strain.

Ancestors

All strains

Escherichia coli K12

Escherichia coli W1485

Escherichia coli W2637

Escherichia coli W3110

Escherichia coli W3110MT

Escherichia coli PHE01

Derived strains

Escherichia coli PHE07

Genotype with respect to wild type

F⁺ (λ) | λ- | inv(rrnD-rrnE) | F-, λ-, rph-1, IN (rrnD, rrnE) | L-tyrosine auxotrophic | Plasmid p15A::pL-aroF-tyrA^1-121-pR-pheA^T326P added

Bars (|) indicate differences between strains.

Plasmids

p15A::pL-aroF-tyrA^1-121-pR-pheA^T326P (p15A ori, pL-aroF-tyrA^1-121-pR-pheA^T326P)

Modifications

Origin	Gene	Organism	Modification
p15A::pL-aroF-tyrA^1-121-pR-pheA^T326P	aroF	Escherichia coli	Overexpression
p15A::pL-aroF-tyrA^1-121-pR-pheA^T326P	tyrA	Escherichia coli	Feedback insensitive
p15A::pL-aroF-tyrA^1-121-pR-pheA^T326P	pheA	Escherichia coli	Feedback insensitive

References

Chen, Minliang; Han, Chao; Zhou, Peng; Shi, Run; Xing, Zhiwei; Chen, Qianqian; Xie, Gou-an; Xie, Rufei; Tan, Wei & Liang, Hengyu. Rational metabolic engineering of Escherichia coli for the industrial-scale production of l-phenylalanine. Bioresource Technology. 2025, 426, 132325.