Escherichia coli EF-18
Ancestors
Genotype with respect to parental
ygaY::Ptrc-yonT (modified)
Genotype with respect to wild type
F+ (λ) | λ- | F- λ- ilvG- rfb-50 rph-1 | △lacI | Plasmid pduet-rib added | △pfkA | △edd△eda | △ribB-FMN riboswitch | ribF::PJ23111-ribCG199D | △purR | ybeM::Ptac-prs-purF | yncI::Ptac-gmk-ndk | ycgH::Ptac-rpiA-rpiB | yghX::Ptac-pgl | ylbE:Ptac-gnd | ilvG::Ptac-yigB | ygaY::Ptrc-yonT (modified) | Plasmid pduet-rib lost | Plasmid placI-rib addedBars (|) indicate differences between strains.
Plasmids
-
placI-rib (lacI, Ptac-ribEDAH)
Modifications
| Origin | Gene | Organism | Modification |
|---|---|---|---|
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Silencing
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Silencing
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Silencing
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Silencing
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Feedback insensitive
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Silencing
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Attenuation
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Silencing
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Silencing
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Overexpression
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Overexpression
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Silencing
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Overexpression
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Overexpression
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Silencing
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Overexpression
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Overexpression
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Silencing
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Overexpression
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Silencing
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Overexpression
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Silencing
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Overexpression
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Silencing
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Overexpression
|
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Overexpression
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Overexpression
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Overexpression
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Overexpression
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Production
| Metabolites | Production type | Production | Biomass | Carbon source | Time | Scale | Ref. |
|---|---|---|---|---|---|---|---|
| riboflavin | Titer | 1287.0 mg/L | Flask | [324] | |||
| riboflavin | Titer | 1970.7 mg/L | Flask | [324] | |||
| riboflavin | Titer | 11.5 g/L | Fed-Batch | [324] | |||
| riboflavin | Substrate yield | 90.4 mg/g of sustrate | Fed-Batch | [324] |
* Inferred from plots using RetroPlot.
Heterologous.
Target metabolites are shown in bold, while non-bold metabolites represent intermediates or potential byproducts.
References
- Zhang, Xiaoling; Li, Yanan; Wang, Kang; Yin, Jilong; Du, Yuxuan; Yang, Zhen; Pan, Xuewei; You, Jiajia & Rao, Zhiming. Construction of antibiotic-free riboflavin producer in Escherichia coli by metabolic engineering strategies with a plasmid stabilization system. Synthetic and Systems Biotechnology. 2025, 10(2), 346-355.