Escherichia coli JHL30
This strain does not exhibit an improved production of the intended metabolite when compared with its closest ancestors.
Ancestors
Genotype with respect to wild type
F+ (λ) | λ- | inv(rrnD-rrnE) | F-, λ-, rph-1, IN (rrnD, rrnE) | ΔatoDA ΔadhE ΔldhA ΔpaaFGH ΔfrdABCD PatoB::BBa_J23100 | Plasmid pBASP addedBars (|) indicate differences between strains.
Plasmids
-
pBASP (PJ23100::crt-PJ23100::hbd-PJ23100::ter-PJ23100::tesB)
Modifications
| Origin | Gene | Organism | Modification |
|---|---|---|---|
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Silencing
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Silencing
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Silencing
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Silencing
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Silencing
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Silencing
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Silencing
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Silencing
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Silencing
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Silencing
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Silencing
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Overexpression
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Overexpression
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Overexpression
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Overexpression
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Overexpression
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Production
| Metabolites | Production type | Production | Biomass | Carbon source | Time | Scale | Ref. |
|---|---|---|---|---|---|---|---|
| butanoate | Titer | 0.8 g/L * | 24 h * | Flask | [353] |
* Inferred from plots using RetroPlot.
Heterologous.
Target metabolites are shown in bold, while non-bold metabolites represent intermediates or potential byproducts.