Escherichia coli HA-2

Ancestors

Genotype with respect to wild type

F⁻ dcm gal ompT hsdS(r_B^- m_B^-) | F⁻ ompT hsdS_B (r_B⁻m_B⁻) gal dcm λ(DE3) | Plasmid pC-Os4CL-HCBT added | Plasmid pA-SeTAL added | Plasmid pETDuet-1 added

Bars (|) indicate differences between strains.

Plasmids

pETDuet-1 (pBR322 origin, P_T7lac-RBS, P_T7lac-RBS, two MCS)

pA-SeTAL (TAL_Se)

pC-Os4CL-HCBT (Os4CL, HCBT)

Modifications

Origin	Gene	Organism	Modification
pA-SeTAL	sam8	Saccharothrix espanaensis	Overexpression
pC-Os4CL-HCBT	4CL	Oryza sativa	Overexpression
pC-Os4CL-HCBT	HCBT2	Dianthus caryophyllus	Overexpression

References

Lee, Su Jin; Sim, Geun Young; Kang, Hyunook; Yeo, Won Seok; Kim, Bong-Gyu & Ahn, Joong-Hoon (2018). Synthesis of avenanthramides using engineered Escherichia coli. Microbial Cell Factories, 17(1).