Escherichia coli HA-5

Ancestors

All strains

Escherichia coli B

Escherichia coli BL21

Escherichia coli BL21(DE3)

Escherichia coli HA-1

Escherichia coli HA-5

Genotype with respect to wild type

F⁻ dcm gal ompT hsdS(r_B^- m_B^-) | F⁻ ompT hsdS_B (r_B⁻m_B⁻) gal dcm λ(DE3) | Plasmid pC-Os4CL-HCBT added | Plasmid pE-trpEG added | Plasmid pA-aroG^fbr-SeTAL-tyrA^fbr added

Bars (|) indicate differences between strains.

Plasmids

pA-aroG^fbr-SeTAL-tyrA^fbr (TAL_Se, aroG^fbr_Ec, tyrA^fbr_Ec)

pC-Os4CL-HCBT (Os4CL, HCBT)

pE-trpEG (trpEG)

Modifications

Origin	Gene	Organism	Modification
pA-aroG^fbr-SeTAL-tyrA^fbr	sam8	Saccharothrix espanaensis	Overexpression
pA-aroG^fbr-SeTAL-tyrA^fbr	aroG	Escherichia coli	Feedback insensitive
pA-aroG^fbr-SeTAL-tyrA^fbr	tyrA	Escherichia coli	Feedback insensitive
pC-Os4CL-HCBT	4CL	Oryza sativa	Overexpression
pC-Os4CL-HCBT	HCBT2	Dianthus caryophyllus	Overexpression
pE-trpEG	trpE	Escherichia coli	Overexpression

References

Lee, Su Jin; Sim, Geun Young; Kang, Hyunook; Yeo, Won Seok; Kim, Bong-Gyu & Ahn, Joong-Hoon (2018). Synthesis of avenanthramides using engineered Escherichia coli. Microbial Cell Factories, 17(1).