L-leucine charts

Silencing

Overexpression

Feedback insensitive

Attenuation

Feed-forward insensitive

Inhibition insensitive

Unstated

Combined modifications

Titer in Fed-Batch for L-leucine

		Modifications
Strain	Maximum production	leuA_Ec	leuB_Ec	leuC_Ec	leuD_Ec	ilvI_Ec	ilvH_Ec	ilvE_Ec	ilvD_Ec	ilvC_Ec	livK_Ec	azlC_Cg	livJ_Ec	brnF_Cg	brnE_Cg	brnQ_Ec	pntA_Ec	pntB_Ec	ldh_Bc	poxB_Ec	pflB_Ec	ldhA_Ec	sucA_Ec	sucB_Ec	trpR_Ec
E. coli LEU23	54,600 mg/L
E. coli LEU27	55,000 mg/L

Titer in Flask for L-leucine

		Modifications
Strain	Maximum production	leuA_Cg	leuA_Ec	leuB_Ec	leuC_Ec	leuD_Ec	ilvI_Ec	ilvH_Ec	ilvE_Ec	ilvD_Ec	ilvC_Ec	livK_Ec	livJ_Ec	brnF_Cg	brnE_Cg	azlC_Cg	brnQ_Ec	pntA_Ec	pntB_Ec	ldh_Bc	poxB_Ec	pflB_Ec	ldhA_Ec	sucA_Ec	sucB_Ec	trpR_Ec	leuE_Ec
E. coli W3110	0 mg/L
E. coli W3110 ΔlacI	0 mg/L
E. coli LEU03	1,300 mg/L
E. coli LEU04	1,900 mg/L
E. coli LEU05	2,100 mg/L
E. coli LEU01	3,000 mg/L
E. coli LEU02	3,550 mg/L
E. coli LEU06	4,800 mg/L
E. coli LEU07	6,270 mg/L
E. coli LEU08	6,440 mg/L
E. coli LEU09	6,800 mg/L
E. coli LEU10	7,350 mg/L
E. coli LEU11	8,000 mg/L
E. coli LEU13	8,230 mg/L
E. coli LEU12	8,450 mg/L
E. coli LEU14	8,740 mg/L
E. coli LEU15	10,150 mg/L
E. coli LEU16	10,760 mg/L
E. coli LEU29	11,150 mg/L
E. coli LEU19	11,260 mg/L
E. coli LEU18	11,620 mg/L
E. coli LEU17	11,800 mg/L
E. coli LEU26	12,660 mg/L
E. coli LEU28	12,660 mg/L
E. coli LEU30	13,040 mg/L
E. coli LEU20	16,100 mg/L
E. coli LEU27	16,250 mg/L
E. coli LEU21	16,570 mg/L
E. coli LEU22	16,690 mg/L
E. coli LEU23	17,150 mg/L

Substrate yield in Fed-Batch for L-leucine

		Modifications
Strain	Maximum production	leuA_Ec	leuB_Ec	leuC_Ec	leuD_Ec	ilvI_Ec	ilvH_Ec	ilvE_Ec	ilvD_Ec	ilvC_Ec	livK_Ec	azlC_Cg	livJ_Ec	brnF_Cg	brnE_Cg	brnQ_Ec	pntA_Ec	pntB_Ec	ldh_Bc	poxB_Ec	pflB_Ec	ldhA_Ec	sucA_Ec	sucB_Ec	trpR_Ec
E. coli LEU23	0.17 g/g of sustrate
E. coli LEU27	0.23 g/g of sustrate

Substrate yield in Flask for L-leucine

		Modifications
Strain	Maximum production	leuA_Ec	leuB_Ec	leuC_Ec	leuD_Ec	ilvI_Ec	ilvH_Ec	ilvE_Ec	ilvD_Ec	ilvC_Ec	livK_Ec	azlC_Cg	livJ_Ec	brnF_Cg	brnE_Cg	brnQ_Ec	pntA_Ec	pntB_Ec	ldh_Bc	poxB_Ec	pflB_Ec	ldhA_Ec	sucA_Ec	sucB_Ec	trpR_Ec	leuA_Cg	leuE_Ec
E. coli W3110	0 g/g of sustrate
E. coli W3110 ΔlacI	0 g/g of sustrate
E. coli LEU03	0.011 g/g of sustrate
E. coli LEU04	0.016 g/g of sustrate
E. coli LEU05	0.018 g/g of sustrate
E. coli LEU01	0.025 g/g of sustrate
E. coli LEU02	0.03 g/g of sustrate
E. coli LEU06	0.04 g/g of sustrate
E. coli LEU07	0.052 g/g of sustrate
E. coli LEU08	0.054 g/g of sustrate
E. coli LEU09	0.057 g/g of sustrate
E. coli LEU10	0.061 g/g of sustrate
E. coli LEU11	0.067 g/g of sustrate
E. coli LEU13	0.069 g/g of sustrate
E. coli LEU12	0.07 g/g of sustrate
E. coli LEU14	0.073 g/g of sustrate
E. coli LEU15	0.085 g/g of sustrate
E. coli LEU16	0.09 g/g of sustrate
E. coli LEU19	0.094 g/g of sustrate
E. coli LEU18	0.097 g/g of sustrate
E. coli LEU17	0.098 g/g of sustrate
E. coli LEU20	0.13 g/g of sustrate
E. coli LEU21	0.14 g/g of sustrate
E. coli LEU22	0.14 g/g of sustrate
E. coli LEU23	0.14 g/g of sustrate
E. coli LEU29	0.14 g/g of sustrate
E. coli LEU26	0.16 g/g of sustrate
E. coli LEU28	0.16 g/g of sustrate
E. coli LEU30	0.16 g/g of sustrate
E. coli LEU27	0.2 g/g of sustrate