Silencing
Overexpression
Feedback insensitive
Attenuation
Feed-forward insensitive
Inhibition insensitive
Unstated
Combined modifications
Titer in Flask for L-isoleucine
| Modifications | |||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Strain | Maximum production | thrAEc | lysCEc | thrBEc | thrCEc | lysAEc | metAEc | ilvAEc | tdhEc | iclREc | ppcEc | ilvIEc | ilvHEc | ygaZEc | ygaHEc | ilvEEc | ilvDEc | ilvCEc | lrpEc |
| E. coli ILE01 (pTacilvAIH, pBRthrABC) | 322 mg/L | ||||||||||||||||||
| E. coli ILE01 (pTacilvAIH, pBRthrABCygaZH) | 1,110 mg/L | ||||||||||||||||||
| E. coli ILE02 (pTacilvAIH, pBRthrABCygaZH) | 2,110 mg/L | ||||||||||||||||||
| E. coli ILE03 (pTacilvAIH, pBRthrABCygaZH) | 2,830 mg/L | ||||||||||||||||||
Titer in Fed-Batch for L-isoleucine
| Modifications | |||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Strain | Maximum production | thrAEc | lysCEc | thrBEc | thrCEc | lysAEc | metAEc | ilvAEc | tdhEc | iclREc | ppcEc | ilvEEc | ilvDEc | ilvCEc | lrpEc | ilvIEc | ilvHEc | ygaZEc | ygaHEc |
| E. coli ILE03 (pTacilvAIH, pBRthrABCygaZH) | 9,460 mg/L | ||||||||||||||||||
Volumetric productivity in Fed-Batch for L-isoleucine
| Modifications | |||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Strain | Maximum production | thrAEc | lysCEc | thrBEc | thrCEc | lysAEc | metAEc | ilvAEc | tdhEc | iclREc | ppcEc | ilvEEc | ilvDEc | ilvCEc | lrpEc | ilvIEc | ilvHEc | ygaZEc | ygaHEc |
| E. coli ILE03 (pTacilvAIH, pBRthrABCygaZH) | 158 mg/L/h | ||||||||||||||||||
Substrate yield in Fed-Batch for L-isoleucine
| Modifications | |||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Strain | Maximum production | thrAEc | lysCEc | thrBEc | thrCEc | lysAEc | metAEc | ilvAEc | tdhEc | iclREc | ppcEc | ilvEEc | ilvDEc | ilvCEc | lrpEc | ilvIEc | ilvHEc | ygaZEc | ygaHEc |
| E. coli ILE03 (pTacilvAIH, pBRthrABCygaZH) | 0.14 g/g of sustrate | ||||||||||||||||||