Lee, Su Jin; Sim, Geun Young; Kang, Hyunook; Yeo, Won Seok; Kim, Bong-Gyu & Ahn, Joong-Hoon (2018). Synthesis of avenanthramides using engineered Escherichia coli. Microbial Cell Factories, 17(1).
Strains
- Escherichia coli BL21(DE3)
- Escherichia coli BtyrR-trpD
- Escherichia coli HA-1
- Escherichia coli HA-2
- Escherichia coli HA-3
- Escherichia coli HA-4
- Escherichia coli HA-5
- Escherichia coli HA-7
- Escherichia coli HA-8
- Escherichia coli HA-Hpa
- Escherichia coli HA-S
- Escherichia coli HA-6
Plasmids
- pA-SeTAL
- pA-aroG-SeTAL-tyrA
- pA-aroGfbr-SeTAL-tyrAfbr
- pA-aroGfbr-ppsA-tktA-SeTAL-tyrAfbr
- pGEX-5X-3
- pG-HpaBC
- pG-SOMT9
- pC-Os4CL
- pC-Os4CL-HCBT
- pE-trpEG
- pETDuet-1
Metabolite production
-
avenanthramide D
-
Titer
- 74.9 mg/L (Escherichia coli HA-4 in Flask)
- 21.4 mg/L (Escherichia coli HA-3 in Flask)
- 34.2 mg/L (Escherichia coli HA-5 in Flask)
- 25.0 mg/L (Escherichia coli HA-6 in Flask)
- 0.0 mg/L (Escherichia coli HA-2 in Flask)
-
L-tyrosine
-
Titer
- 0.0 mg/L (Escherichia coli HA-2 in Flask)
- 0.0 mg/L (Escherichia coli HA-3 in Flask)
- 4.08 mg/L (Escherichia coli HA-4 in Flask)
- 611.52 mg/L (Escherichia coli HA-5 in Flask)
- 527.49 mg/L (Escherichia coli HA-6 in Flask)
-
4-coumarate
-
Titer
- 13.78 mg/L (Escherichia coli HA-2 in Flask)
- 0.0 mg/L (Escherichia coli HA-3 in Flask)
- 2.52 mg/L (Escherichia coli HA-4 in Flask)
- 124.22 mg/L (Escherichia coli HA-5 in Flask)
- 105.45 mg/L (Escherichia coli HA-6 in Flask)
-
anthranilate
-
Titer
- 0.0 mg/L (Escherichia coli HA-2 in Flask)
- 12.17 mg/L (Escherichia coli HA-3 in Flask)
- 12.17 mg/L (Escherichia coli HA-4 in Flask)
- 5.73 mg/L (Escherichia coli HA-5 in Flask)
- 0.9 mg/L (Escherichia coli HA-6 in Flask)